human umbilical vein endothelial cells huvec tert2 endothelial cells  (ATCC)

Journal: Archives of Toxicology

Article Title: Transcriptomic-based evaluation of trichloroethylene glutathione and cysteine conjugates demonstrate phenotype-dependent stress responses in a panel of human in vitro models

doi: 10.1007/s00204-022-03436-6

Figure Lengend Snippet: Diagram of mercapturic acid pathway of trichloroethylene (TCE) and human cell models tested. Enzymes involved: a glutathione S-transferases; b γ-glutamyltransferase; c cysteinyl-glycine dipeptidase; d cysteine conjugate β-lyase; e cysteine conjugate N-acetyltransferase; f aminoacylase. Formation of sulfoxides of cysteine conjugates and mercapturic acids is not shown to avoid an overcomplicated figure. The tested chemicals in this study were 1,2-DCVG, 2,2-DCVG, 1,2-DCVC and 2,2-DCVC. Human in vitro cell models tested: HUVEC/TERT2 as the endothelial vascular system, HepaRG as a hepatic model, RPTEC/TERT1 as a renal model, LUHMES as dopaminergic neurons model, UKN5 as hiPSC derived peripheral neurons and BCC42, as hiPSC derived brain cortical cultures containing all subtypes of neurons and astrocytes

Article Snippet: HUVEC/TERT2, (Evercyte GmbH, Vienna, Austria) human umbilical vein endothelial cells were cultivated in EGM Endothelial Cell Growth Medium (Lonza CC-3124) supplemented with Endothelial Cell Growth Medium SingleQuots Supplements (CC-4133): endothelial growth supplement (EnG), hEGF, hydrocortisone, ascorbic acid and heparin plus 10% Fetal Bovine Serum (FBS).

Techniques: In Vitro, Derivative Assay